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[pkgsrc/trunk]: pkgsrc/biology/kallisto biology/kallisto: import kallisto-0.45.0



details:   https://anonhg.NetBSD.org/pkgsrc/rev/3ddea89698cf
branches:  trunk
changeset: 389167:3ddea89698cf
user:      bacon <bacon%pkgsrc.org@localhost>
date:      Fri Dec 21 19:00:56 2018 +0000

description:
biology/kallisto: import kallisto-0.45.0

Kallisto is a program for quantifying abundances of transcripts from RNA-Seq
data, or more generally of target sequences using high-throughput sequencing
reads. It is based on the novel idea of pseudoalignment for rapidly determining
the compatibility of reads with targets, without the need for alignment.

diffstat:

 biology/kallisto/DESCR                            |   4 ++
 biology/kallisto/Makefile                         |  38 +++++++++++++++++++
 biology/kallisto/PLIST                            |  10 +++++
 biology/kallisto/distinfo                         |   8 ++++
 biology/kallisto/files/kallisto-test              |  45 +++++++++++++++++++++++
 biology/kallisto/patches/patch-CMakeLists.txt     |  28 ++++++++++++++
 biology/kallisto/patches/patch-src_CMakeLists.txt |  30 +++++++++++++++
 7 files changed, 163 insertions(+), 0 deletions(-)

diffs (191 lines):

diff -r 8c536bdc9407 -r 3ddea89698cf biology/kallisto/DESCR
--- /dev/null   Thu Jan 01 00:00:00 1970 +0000
+++ b/biology/kallisto/DESCR    Fri Dec 21 19:00:56 2018 +0000
@@ -0,0 +1,4 @@
+Kallisto is a program for quantifying abundances of transcripts from RNA-Seq
+data, or more generally of target sequences using high-throughput sequencing
+reads. It is based on the novel idea of pseudoalignment for rapidly determining
+the compatibility of reads with targets, without the need for alignment.
diff -r 8c536bdc9407 -r 3ddea89698cf biology/kallisto/Makefile
--- /dev/null   Thu Jan 01 00:00:00 1970 +0000
+++ b/biology/kallisto/Makefile Fri Dec 21 19:00:56 2018 +0000
@@ -0,0 +1,38 @@
+# $NetBSD: Makefile,v 1.1 2018/12/21 19:00:56 bacon Exp $
+
+DISTNAME=      kallisto-${PV}
+CATEGORIES=    biology
+MASTER_SITES=  ${MASTER_SITE_GITHUB:=pachterlab/}
+GITHUB_TAG=    v${PV}
+
+MAINTAINER=    bacon%NetBSD.org@localhost
+HOMEPAGE=      https://pachterlab.github.io/kallisto/
+COMMENT=       Quantify abundances of transcripts from RNA-Seq data
+LICENSE=       2-clause-bsd
+
+USE_LANGUAGES= c c++
+USE_TOOLS+=    pax
+USE_CMAKE=     yes
+
+SUBST_CLASSES+=                test
+SUBST_STAGE.test=      post-build
+SUBST_FILES.test=      kallisto-test
+SUBST_SED.test=                -e 's|/usr/local/share/kallisto|${DATADIR}|g'
+
+PV=            0.45.0
+DATADIR=       ${PREFIX}/share/kallisto
+
+INSTALLATION_DIRS=     bin ${DATADIR}
+
+post-extract:
+       ${CP} ${FILESDIR}/kallisto-test ${WRKSRC}
+
+post-install:
+       ${INSTALL_SCRIPT} ${WRKSRC}/kallisto-test ${DESTDIR}${PREFIX}/bin
+       cd ${WRKSRC} && pax -rw test ${DESTDIR}${DATADIR}
+
+.include "../../biology/htslib/buildlink3.mk"
+.include "../../devel/hdf5/buildlink3.mk"
+.include "../../archivers/szip/buildlink3.mk"
+.include "../../devel/zlib/buildlink3.mk"
+.include "../../mk/bsd.pkg.mk"
diff -r 8c536bdc9407 -r 3ddea89698cf biology/kallisto/PLIST
--- /dev/null   Thu Jan 01 00:00:00 1970 +0000
+++ b/biology/kallisto/PLIST    Fri Dec 21 19:00:56 2018 +0000
@@ -0,0 +1,10 @@
+@comment $NetBSD: PLIST,v 1.1 2018/12/21 19:00:56 bacon Exp $
+bin/kallisto
+bin/kallisto-test
+share/kallisto/test/README.md
+share/kallisto/test/Snakefile
+share/kallisto/test/chrom.txt
+share/kallisto/test/reads_1.fastq.gz
+share/kallisto/test/reads_2.fastq.gz
+share/kallisto/test/transcripts.fasta.gz
+share/kallisto/test/transcripts.gtf.gz
diff -r 8c536bdc9407 -r 3ddea89698cf biology/kallisto/distinfo
--- /dev/null   Thu Jan 01 00:00:00 1970 +0000
+++ b/biology/kallisto/distinfo Fri Dec 21 19:00:56 2018 +0000
@@ -0,0 +1,8 @@
+$NetBSD: distinfo,v 1.1 2018/12/21 19:00:56 bacon Exp $
+
+SHA1 (kallisto-0.45.0.tar.gz) = ab0890c5d675d678dbcf415a86512b4694b3a4a6
+RMD160 (kallisto-0.45.0.tar.gz) = f482f7525dde0bfc2425cf803812c1c7748c882e
+SHA512 (kallisto-0.45.0.tar.gz) = b120c36ccb4725b19307c3e90c7f9b73b87efc53f08d3f2c12fc2e4b7afc3c03c52ba07dff623c9c8ad5a7e89c1eeb907389ccf3e4440236e81a0611500639e2
+Size (kallisto-0.45.0.tar.gz) = 2250430 bytes
+SHA1 (patch-CMakeLists.txt) = 2817091347e35898fec0bab77e8d9cc5ca5fff3f
+SHA1 (patch-src_CMakeLists.txt) = 2ef72024591c5216fbf44fb7cdfa4a8330b0c81a
diff -r 8c536bdc9407 -r 3ddea89698cf biology/kallisto/files/kallisto-test
--- /dev/null   Thu Jan 01 00:00:00 1970 +0000
+++ b/biology/kallisto/files/kallisto-test      Fri Dec 21 19:00:56 2018 +0000
@@ -0,0 +1,45 @@
+#!/bin/sh
+
+##########################################################################
+#   Script description:
+#       Run standard kallisto test data
+#       
+#   History:
+#   Date        Name        Modification
+#   2016-03-21  J Bacon     Begin
+##########################################################################
+
+usage()
+{
+    printf "Usage: $0 test-directory\n"
+    exit 1
+}
+
+
+##########################################################################
+#   Main
+##########################################################################
+
+if [ $# != 1 ]; then
+    usage
+fi
+
+dir="$1"
+if [ -e "$dir" ]; then
+    printf "$dir already exists.  Remove it first or choose a different name.\n"
+    exit 1
+fi
+
+cp -iR /usr/local/share/kallisto/test/ "$dir"
+cd "$dir"
+kallisto index -i transcripts.idx transcripts.fasta.gz
+kallisto quant -i transcripts.idx -o output -b 100 reads_1.fastq.gz reads_2.fastq.gz
+ls -l output
+more output/abundance.tsv
+
+cat << EOM
+
+See https://pachterlab.github.io/kallisto/starting.html for instructions
+on interpreting the output above.
+
+EOM
diff -r 8c536bdc9407 -r 3ddea89698cf biology/kallisto/patches/patch-CMakeLists.txt
--- /dev/null   Thu Jan 01 00:00:00 1970 +0000
+++ b/biology/kallisto/patches/patch-CMakeLists.txt     Fri Dec 21 19:00:56 2018 +0000
@@ -0,0 +1,28 @@
+$NetBSD: patch-CMakeLists.txt,v 1.1 2018/12/21 19:00:56 bacon Exp $
+
+# Unbundle htslib
+
+--- CMakeLists.txt.orig        2018-12-19 17:13:04.000000000 +0000
++++ CMakeLists.txt
+@@ -35,21 +35,6 @@ ELSE(LINK MATCHES shared)
+ ENDIF(LINK MATCHES static)
+ 
+ 
+-include(ExternalProject)
+-ExternalProject_Add(htslib
+-    PREFIX ${PROJECT_SOURCE_DIR}/ext/htslib
+-    SOURCE_DIR ${PROJECT_SOURCE_DIR}/ext/htslib
+-    BUILD_IN_SOURCE 1
+-    CONFIGURE_COMMAND autoheader && autoconf && ${PROJECT_SOURCE_DIR}/ext/htslib/configure 
+-        --prefix=${PREFIX} --disable-bz2 --disable-lzma --disable-libcurl
+-    BUILD_COMMAND make lib-static
+-    INSTALL_COMMAND ""
+-)
+-
+-include_directories(${htslib_PREFIX}/src/htslib)
+-
+-
+-
+ # add_compile_options(-Wdeprecated-register)
+ 
+ add_subdirectory(src)
diff -r 8c536bdc9407 -r 3ddea89698cf biology/kallisto/patches/patch-src_CMakeLists.txt
--- /dev/null   Thu Jan 01 00:00:00 1970 +0000
+++ b/biology/kallisto/patches/patch-src_CMakeLists.txt Fri Dec 21 19:00:56 2018 +0000
@@ -0,0 +1,30 @@
+$NetBSD: patch-src_CMakeLists.txt,v 1.1 2018/12/21 19:00:56 bacon Exp $
+
+# Unbundle htslib
+
+--- src/CMakeLists.txt.orig    2018-10-17 23:58:22 UTC
++++ src/CMakeLists.txt
+@@ -3,15 +3,13 @@ file(GLOB headers *.h *.hpp)
+ 
+ list(REMOVE_ITEM sources main.cpp)
+ 
+-include_directories(../ext/htslib)
+-
+ add_library(kallisto_core ${sources} ${headers})
+ target_include_directories(kallisto_core PUBLIC ${CMAKE_CURRENT_SOURCE_DIR})
+ 
+ add_executable(kallisto main.cpp)
+ 
+ find_package( Threads REQUIRED )
+-target_link_libraries(kallisto kallisto_core pthread ${CMAKE_CURRENT_SOURCE_DIR}/../ext/htslib/libhts.a)
++target_link_libraries(kallisto kallisto_core pthread hts)
+ 
+ if(LINK MATCHES static)
+     set(BUILD_SHARED_LIBS OFF)
+@@ -56,4 +54,4 @@ else()
+ endif(LINK MATCHES static)
+ 
+ 
+-install(TARGETS kallisto DESTINATION "${CMAKE_INSTALL_BINDIR}")
+\ No newline at end of file
++install(TARGETS kallisto DESTINATION "${CMAKE_INSTALL_BINDIR}")



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