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CVS commit: pkgsrc/biology/samtools
Module Name: pkgsrc
Committed By: adam
Date: Tue Nov 6 10:49:41 UTC 2018
Modified Files:
pkgsrc/biology/samtools: Makefile distinfo
Log Message:
samtools: updated to 1.9
Release 1.9:
* Samtools mpileup VCF and BCF output is now deprecated. It is still
functional, but will warn. Please use bcftools mpileup instead.
* Samtools mpileup now handles the '-d' max_depth option differently. There
is no longer an enforced minimum, and '-d 0' is interpreted as limitless
(no maximum - warning this may be slow). The default per-file depth is
now 8000, which matches the value mpileup used to use when processing
a single sample. To get the previous default behaviour use the higher
of 8000 divided by the number of samples across all input files, or 250.
* Samtools stats new features:
- The '--remove-overlaps' option discounts overlapping portions of
templates when computing coverage and mapped base counting.
- When a target file is in use, the number of bases inside the
target is printed and the percentage of target bases with coverage
above a given threshold specified by the '--cov-threshold' option.
- Split base composition and length statistics by first and last reads.
* Samtools faidx new features:
- Now takes long options.
- Now warns about zero-length and truncated sequences due to the
requested range being beyond the end of the sequence.
- Gets a new option (--continue) that allows it to carry on
when a requested sequence was not in the index.
- It is now possible to supply the list of regions to output in a text
file using the new '--region-file' option.
- New '-i' option to make faidx return the reverse complement of
the regions requested.
- faidx now works on FASTQ (returning FASTA) and added a new
fqidx command to index and return FASTQ.
* Samtools collate now has a fast option '-f' that only operates on
primary pairs, dropping secondary and supplementary. It tries to write
pairs to the final output file as soon as both reads have been found.
* Samtools bedcov gets a new '-j' option to make it ignore deletions (D) and
reference skips (N) when computing coverage.
* Small speed up to samtools coordinate sort, by converting it to use
radix sort.
* Samtools idxstats now works on SAM and CRAM files, however this
isn't fast due to some information lacking from indices.
* Compression levels may now be specified with the level=N
output-fmt-option. E.g. with -O bam,level=3.
* Various documentation improvements.
* Bug-fixes:
- Improved error reporting in several places.
- Various test improvements.
- Fixed failures in the multi-region iterator (view -M) when regions
provided via BED files include overlaps
- Samtools stats now counts '=' and 'X' CIGAR operators when
counting mapped bases.
- Samtools stats has fixes for insert size filtering (-m, -i).
- Samtools stats -F now longer negates an earlier -d option.
- Fix samtools stats crash when using a target region.
- Samtools sort now keeps to a single thread when the -@ option is absent.
Previously it would spawn a writer thread, which could cause the CPU
usage to go slightly over 100%.
- Fixed samtools phase '-A' option which was incorrectly defined to take
a parameter.
- Fixed compilation problems when using C_INCLUDE_PATH.
- Fixed --version when built from a Git repository.
- Use noenhanced mode for title in plot-bamstats. Prevents unwanted
interpretation of characters like underscore in gnuplot version 5.
- blast2sam.pl now reports perfect match hits (no indels or mismatches).
- Fixed bug in fasta and fastq subcommands where stdout would not be flushed
correctly if the -0 option was used.
- Fixed invalid memory access in mpileup and depth on alignment records
where the sequence is absent.
To generate a diff of this commit:
cvs rdiff -u -r1.2 -r1.3 pkgsrc/biology/samtools/Makefile
cvs rdiff -u -r1.1 -r1.2 pkgsrc/biology/samtools/distinfo
Please note that diffs are not public domain; they are subject to the
copyright notices on the relevant files.
Modified files:
Index: pkgsrc/biology/samtools/Makefile
diff -u pkgsrc/biology/samtools/Makefile:1.2 pkgsrc/biology/samtools/Makefile:1.3
--- pkgsrc/biology/samtools/Makefile:1.2 Wed Aug 22 09:42:59 2018
+++ pkgsrc/biology/samtools/Makefile Tue Nov 6 10:49:41 2018
@@ -1,26 +1,27 @@
-# $NetBSD: Makefile,v 1.2 2018/08/22 09:42:59 wiz Exp $
+# $NetBSD: Makefile,v 1.3 2018/11/06 10:49:41 adam Exp $
-DISTNAME= samtools-1.8
-PKGREVISION= 1
+DISTNAME= samtools-1.9
CATEGORIES= biology
MASTER_SITES= ${MASTER_SITE_GITHUB:=samtools/}
-GITHUB_TAG= ${PKGVERSION_NOREV}
+EXTRACT_SUFX= .tar.bz2
+GITHUB_RELEASE= ${PKGVERSION_NOREV}
MAINTAINER= bacon%NetBSD.org@localhost
HOMEPAGE= http://www.htslib.org/
COMMENT= Tools for manipulating next-generation sequencing data
LICENSE= mit
-USE_TOOLS+= autoconf automake autoreconf gmake perl:run
-GNU_CONFIGURE= yes
-REPLACE_PERL= misc/*.pl misc/plot-bamstats test/test.pl
-REPLACE_PYTHON= misc/*.py
+USE_TOOLS+= gmake perl:run
+GNU_CONFIGURE= yes
+CONFIGURE_ARGS+= --with-htslib=system
+TEST_TARGET= test
-pre-configure:
- cd ${WRKSRC} && autoreconf
+REPLACE_PERL= misc/*.pl
+REPLACE_PERL+= misc/plot-bamstats
+REPLACE_PERL+= test/test.pl
+REPLACE_PYTHON= misc/*.py
-.include "../../mk/curses.buildlink3.mk"
-.include "../../lang/python/application.mk"
.include "../../biology/htslib/buildlink3.mk"
-.include "../../devel/zlib/buildlink3.mk"
+.include "../../lang/python/application.mk"
+.include "../../mk/curses.buildlink3.mk"
.include "../../mk/bsd.pkg.mk"
Index: pkgsrc/biology/samtools/distinfo
diff -u pkgsrc/biology/samtools/distinfo:1.1 pkgsrc/biology/samtools/distinfo:1.2
--- pkgsrc/biology/samtools/distinfo:1.1 Mon May 7 18:37:31 2018
+++ pkgsrc/biology/samtools/distinfo Tue Nov 6 10:49:41 2018
@@ -1,6 +1,6 @@
-$NetBSD: distinfo,v 1.1 2018/05/07 18:37:31 bacon Exp $
+$NetBSD: distinfo,v 1.2 2018/11/06 10:49:41 adam Exp $
-SHA1 (samtools-1.8.tar.gz) = 5ac70ba4c324c223398ab4c0689933564985303c
-RMD160 (samtools-1.8.tar.gz) = 47e4fde82a686669322fd330374aa37b148b7954
-SHA512 (samtools-1.8.tar.gz) = 82e4d5f855f944cc989902154a99eada2b4c81939bd936fa1d471cf9ff01e03c8a7f773928ae55ef691f2827aa4076126678940bb89a322fa56d83c517677d0c
-Size (samtools-1.8.tar.gz) = 4047378 bytes
+SHA1 (samtools-1.9.tar.bz2) = 267191dc2f5d24eb0b22c0094138270ed5384241
+RMD160 (samtools-1.9.tar.bz2) = b7f49e1bdd569f63ec53330f43df7e5566ba8a16
+SHA512 (samtools-1.9.tar.bz2) = 6c8207754615ae8dad84b278f93e7d57eb40b818efb80f6aff6aba22fde5772247724e86596e765791e3175ffbb683393dd3ddfe7681c73eef4eb4f2342bf68f
+Size (samtools-1.9.tar.bz2) = 4440405 bytes
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